A new method has been formulated and validated to analyse levels of dihydroxyacetone in floral nectar of Manuka (Leptospermum scoparium). The new method was then used on samples collected for regions across New Zealand, in both the North Island and the Nelson region of the upper South Island between 2009-2012, along with some Australian samples. The tests concluded that the ration of dihydroxyacetone to total sugar (DHA/Tsugar) was considered as low as (0.002 mg/mg). Inter and intraregional variation was also observed, alongside inter annual variation with variation from low to high classification occurring within a region and from low to moderate over time. Australian species also exhibited elevated levels of dihydroxyacetone in the nectar. Some garden cultivars proved to produce high nectar DHA/Tsugar, and a survey of cultivars was taken; cultivars with single flowered red or pink flowers proved to produce the highest nectar DHA/Tsugar levels.